Endotoxin testing of a wound debridement device containing medicinal Lucilia sericata larvae

Alimentary products of medicinal Lucilia sericata larvae are studied to determine their mechanisms of action, particularly in the contexts of wound debridement and disinfection. Furthermore, the larvae can be applied to patients in contained devices, such as the BioBag (BioMonde). Here, we tested the materials and larval content of the most commonly used BioBag (the “BB-50”) to explore the possibility that endotoxins may be contributing to the bio-activity of the product, given that endotoxins are potent stimulants of cellular activation. Using standardised protocols to collect larval alimentary products (LAP), we proceeded to determine residual endotoxin levels in LAP derived from the BioBag, before and after the neutralisation of interfering enzymatic activity. The BB-50 device and its associated larval content was not a significant source of LPS activity. However, it is clear from these experiments that a failure to remove the confounding serine proteinase activity would have resulted in spuriously high and erroneous results. The residual LPS levels detected are unlikely to be active in wound healing assays, following cross-referencing to publications where LPS at much higher levels has been shown to have positive and negative effects on processes associated with wound repair and tissue regeneration

Haematophagic Caenorhabditis elegans

Caenorhabditis elegans is a free-living nematode that resides in soil and typically feeds on bacteria. We postulate that haematophagic C. elegans could provide a model to evaluate vaccine responses to intestinal proteins from hematophagous nematode parasites, such as Necator americanus. Human erythrocytes, fluorescently labelled with tetramethylrhodamine succinimidyl ester, demonstrated a stable bright emission and facilitated visualization of feeding events with fluorescent microscopy. C. elegans were observed feeding on erythrocytes and were shown to rupture red blood cells upon capture to release and ingest their contents. In addition, C. elegans survived equally on a diet of erythrocytes. There was no statistically significant difference in survival when compared with a diet of Escherichia coli OP50. The enzymes responsible for the digestion and detoxification of haem and haemoglobin, which are key components of the hookworm vaccine, were found in the C. elegans intestine. These findings support our postulate that free-living nematodes could provide a model for the assessment of neutralizing antibodies to current and future hematophagous parasite vaccine candidates

Unsteady flow of a thixotropic fluid in a slowly varying pipe

We analyse the unsteady axisymmetric flow of a thixotropic or antithixotropic fluid in a slowly varying cylindrical pipe. We derive general perturbation solutions in regimes of small Deborah numbers, in which thixotropic or antithixotropic effects enter as perturbations to generalised Newtonian flow. We present results for the viscous Moore–Mewis–Wagner model and the viscoplastic Houška model, and we use these results to elucidate what can be predicted in general about the behaviour of thixotropic and antithixotropic fluids in lubrication flow. The range of behaviour we identify casts doubt on the efficacy of model reduction approaches that postulate a generic cross-pipe flow structure

Bragg Scattering as a Probe of Atomic Wavefunctions and Quantum Phase Transitions in Optical Lattices

We have observed Bragg scattering of photons from quantum degenerate $^{87}$Rb atoms in a three-dimensional optical lattice. Bragg scattered light directly probes the microscopic crystal structure and atomic wavefunction whose position and momentum width is Heisenberg-limited. The spatial coherence of the wavefunction leads to revivals in the Bragg scattered light due to the atomic Talbot effect. The decay of revivals across the superfluid to Mott insulator transition indicates the loss of superfluid coherence.Comment: 5 pages, 4 figure

Double-impulse magnetic focusing of launched cold atoms.

We have theoretically investigated three-dimensional focusing of a launched cloud of cold atoms using a pair of magnetic lens pulses (the alternate-gradient method). Individual lenses focus radially and defocus axially or vice versa. The performance of the two possible pulse sequences are compared and found to be ideal for loading both 'pancake' and 'sausage' shaped magnetic/optical microtraps. It is shown that focusing aberrations are considerably smaller for double-impulse magnetic lenses compared to single-impulse magnetic lenses. An analysis of clouds focused by the double-impulse technique is presented

Atom trapping with a thin magnetic film

We have created a $^{87}$Rb Bose-Einstein condensate in a magnetic trapping potential produced by a hard disk platter written with a periodic pattern. Cold atoms were loaded from an optical dipole trap and then cooled to BEC on the surface with radiofrequency evaporation. Fragmentation of the atomic cloud due to imperfections in the magnetic structure was observed at distances closer than 40 $\mu$m from the surface. Attempts to use the disk as an atom mirror showed dispersive effects after reflection.Comment: 4 pages, 5 figure

Toward collaborative open data science in metabolomics using Jupyter Notebooks and cloud computing

Background A lack of transparency and reporting standards in the scientific community has led to increasing and widespread concerns relating to reproduction and integrity of results. As an omics science, which generates vast amounts of data and relies heavily on data science for deriving biological meaning, metabolomics is highly vulnerable to irreproducibility. The metabolomics community has made substantial efforts to align with FAIR data standards by promoting open data formats, data repositories, online spectral libraries, and metabolite databases. Open data analysis platforms also exist; however, they tend to be inflexible and rely on the user to adequately report their methods and results. To enable FAIR data science in metabolomics, methods and results need to be transparently disseminated in a manner that is rapid, reusable, and fully integrated with the published work. To ensure broad use within the community such a framework also needs to be inclusive and intuitive for both computational novices and experts alike. Aim of Review To encourage metabolomics researchers from all backgrounds to take control of their own data science, mould it to their personal requirements, and enthusiastically share resources through open science. Key Scientific Concepts of Review This tutorial introduces the concept of interactive web-based computational laboratory notebooks. The reader is guided through a set of experiential tutorials specifically targeted at metabolomics researchers, based around the Jupyter Notebook web application, GitHub data repository, and Binder cloud computing platform

Thixotropic pumping in a cylindrical pipe

We consider the flow of a thixotropic fluid in a uniform cylindrical pipe, driven by an oscillating pressure gradient or a body force. For a variety of rheological models, solutions can be obtained by integrating ordinary rather than partial differential equations: we illustrate this approach for the thixo-viscoplastic Houška model and the thixo-viscous simplified Moore-Mewis-Wagner model. We present asymptotic results in the limits of small and large Deborah numbers, and numerical results for intermediate Deborah numbers. Under asymmetrical "sawtooth" forcing, thixotropy leads to the net transport of fluid along the pipe, even when there would be no net transport of the corresponding generalised-Newtonian fluid. We propose the name "thixotropic pumping" for this novel transport mechanism

Atomic Resonance and Scattering

Contains research objectives.Joint Services Electronics Programs (U. S. Army, U.S. Navy, and U.S. Air Force) under Contract DA 36-039-AMC-03200(E)Sloan Fund for Basic Research (M.I.T. Grant 95

Improving effect of metal and oxide nanoparticles encapsulated in porous silica on fermentative biohydrogen production by Clostridium butyricum.

peer reviewedaudience: researcher, professional, student, popularizationThis paper investigated the enhancement effect of nanometre-sized metallic (Pd, Ag and Cu) or metallic oxide (Fe(x)O(y)) nanoparticles on fermentative hydrogen production from glucose by a Clostridium butyricum strain. These nanoparticles (NP) of about 2-3nm were encapsulated in porous silica (SiO(2)) and were added at very low concentration (10(-6)molL(-1)) in batch hydrogen production test. The cultures containing iron oxide NP produced 38% more hydrogen with a higher maximum H(2) production rate (HPR) of 58% than those without NP or with silica particles only. The iron oxide NP were used in a 2.5L sequencing-batch reactor and showed no significant effect on the yields (established at 2.2mol(hydrogen)mol(glucose)(-1)) but an improvement of the HPR (+113%, reaching a maximum HPR of 86mL(hydrogen)L(-1)h(-1)). These results suggest an improvement of the electron transfers trough some combinations between enzymatic activity and inorganic materials.Etude de la production d'hydrogène par les bactéries anaérobies chimiotrophes (dark-fermentation